Publications

Analysis of α-synuclein species enriched from cerebral cortex of humans with sporadic dementia with Lewy bodies.

2020

Sanderson JB, De S, Jiang H, Rovere M, Jin M, Zaccagnini L, Hays Watson A, De Boni L, Lagomarsino VN, Young-Pearse TL, Liu X, Pochapsky TC, Hyman BT, Dickson DW, Klenerman D, Selkoe DJ, Bartels T.

Brain Commun.

Digital Sensing and Molecular Computation by an Enzyme-Free DNA Circuit.

2020

Arter WE, Yusim Y, Peter Q, Taylor CG, Klenerman D, Keyser UF, Knowles TPJ

ACS Nano.

The molecular processes underpinning prion-like spreading and seed amplification in protein aggregation.

2020

Meisl G, Knowles TP, Klenerman D.

Curr Opin Neurobiol.

The Costs of Close Contacts: Visualizing the Energy Landscape of Cell Contacts at the Nanoscale.

2020

Kulenkampff K, Lippert AH, McColl J, Santos AM, Ponjavic A, Jenkins E, Humphrey J, Winkel A, Franze K, Lee SF, Davis SJ, Klenerman D.

Biophys J.

Beta amyloid aggregates induce sensitised TLR4 signalling causing long-term potentiation deficit and rat neuronal cell death.

2020

Hughes C, Choi ML, Yi JH, Kim SC, Drews A, George-Hyslop PS, Bryant C, Gandhi S, Cho K, Klenerman D.

Commun Biol.

MyD88 Death-Domain Oligomerization Determines Myddosome Architecture: Implications for Toll-like Receptor Signaling.

2020

Moncrieffe MC, Bollschweiler D, Li B, Penczek PA, Hopkins L, Bryant CE, Klenerman D, Gay NJ.

Structure

A General in Vitro Assay for Studying Enzymatic Activities of the Ubiquitin System.

2020

Zuo Y, Chong BK, Jiang K, Finley D, Klenerman D, Ye Y.

Biochemistry

α-Synuclein strains target distinct brain regions and cell types.

2020

Lau A, So RWL, Lau HHC, Sang JC, Ruiz-Riquelme A, Fleck SC, Stuart E, Menon S, Visanji NP, Meisl G, Faidi R, Marano MM, Schmitt-Ulms C, Wang Z, Fraser PE, Tandon A, Hyman BT, Wille H, Ingelsson M, Klenerman D, Watts JC.

Nat Neurosci.

N-Terminal Ubiquitination of Amyloidogenic Proteins Triggers Removal of Their Oligomers by the Proteasome Holoenzyme

2020

Ye Y, Klenerman D, Finley D.

J Mol Biol.

Nanoscale-targeted patch-clamp recordings of functional presynaptic ion channels.

2013

P Novak, J Gorelik, U Vivekananda, AI Shevchuk, YS Ermolyuk, RJ Bailey, AJ Bushby, GWJ Moss, DA Rusakov, D Klenerman, DM Kullmann, KE Volynski, YE Korchev

Neuron

Local delivery of molecules from a nanopipette for quantitative receptor mapping on live cells

2013

B Babakinejad, P Jönsson, A López Córdoba, P Actis, P Novak, Y Takahashi, A Shevchuk, U Anand, P Anand, A Drews, A Ferrer-Montiel, D Klenerman, YE Korchev

Analytical Chemistry

A Single gD Glycoprotein Can Mediate Infection by Herpes simplex Virus

2013

RW Clarke, A Drews, H Browne, D Klenerman

Journal of the American Chemical Society

Ultrarapid Generation of Femtoliter Microfluidic Droplets for Single-Molecule-Counting Immunoassays

2013

J-U Shim, RT Ranasinghe, CA Smith, SM Ibrahim, F Hollfelder, WTS Huck, D Klenerman, C Abell

ACS Nano

A Single Molecule Study of Toll-Like-Receptor 4 Structure and Signalling

2013

SL Latty, K Ganzinger, LJ Hopkins, C Bryant, D Klenerman

EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS

Single molecule studies of protein aggregates

2013

D Klenerman

EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS

Using single molecule techniques to find out how the major protein associated with Parkinson's disease

2013

MH Horrocks, L Tosatto, N Cremades, CM Dobson, D Klenerman

EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS

Molecular nanomechanics and local stimulus of individual biomolecules on the surface of cells

2013

P Joensson, AL Cordoba, B Babakinejad, A Drews, SJ Davis, YE Korchev, D Klenerman

EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS

Single molecule fluorescence approach to investigate alpha-synuclein oligomer formation

2013

L Tosatto, MH Horrocks, N Cremades, T Guilliams, M Dalla Serra, D Klenerman

EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS

Single-molecule measurements of transient biomolecular complexes through microfluidic dilution

2013

MH Horrocks, L Rajah, P Jönsson, M Kjaergaard, M Vendruscolo, TPJ Knowles, D Klenerman

Analytical chemistry

Highly Rapid Amplification-Free and Quantitative DNA Imaging Assay

2013

T Klamp, M Camps, B Nieto, F Guasch, RT Ranasinghe, J Wiedemann, Z Petrášek, P Schwille, D Klenerman, M Sauer

Scientific reports

Three-Dimensional RNA Structure of the Major HIV-1 Packaging Signal Region

2013

JD Stephenson, H Li, JC Kenyon, M Symmons, D Klenerman, AML Lever

Structure

Single molecule characterization of the interactions between amyloid-β peptides and the membranes of hippocampal cells

2013

P Narayan, KA Ganzinger, J McColl, L Weimann, S Meehan, S Qamar, JA Carver, MR Wilson, P St George-Hyslop, CM Dobson, D Klenerman

J Am Chem Soc

Imaging the cell surface and its organization down to the level of single molecules.

2013

D Klenerman, A Shevchuk, P Novak, YE Korchev, SJ Davis

Philosophical transactions of the Royal Society of London. Series B, Biological sciences

A quantitative comparison of single-dye tracking analysis tools using Monte Carlo simulations.

2013

L Weimann, KA Ganzinger, J McColl, KL Irvine, SJ Davis, NJ Gay, CE Bryant, D Klenerman

PloS one

Imaging the cell surface and its organization down to the level of single molecules

2013

D Klenerman, A Shevchuk, P Novak, YE Korchev, SJ Davis

Philosophical Transactions of the Royal Society B: Biological Sciences

Megahertz-generated femtoliter microfluidic droplets for single-molecule-counting immunoassay

2013

JU Shim, RT Ranasinghe, F Hollfelder, WTS Huck, D Klenerman, C Abell, J Cooper

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013

Nanoscale-targeted patch-clamp recordings of functional presynaptic ion channels.

2013

P Novak, J Gorelik, U Vivekananda, AI Shevchuk, YS Ermolyuk, RJ Bailey, AJ Bushby, GWJ Moss, DA Rusakov, D Klenerman, DM Kullmann, KE Volynski, YE Korchev

Neuron

Three-dimensional RNA structure of the major HIV-1 packaging signal region

2013

JD Stephenson, H Li, JC Kenyon, M Symmons, D Klenerman, AML Lever

Structure

Ubiquitin chain conformation regulates recognition and activity of interacting proteins

2012

Y Ye, G Blaser, MH Horrocks, MJ Ruedas-Rama, S Ibrahim, AA Zhukov, A Orte, D Klenerman, SE Jackson, D Komander

Nature

Pipette–Surface Interaction: Current Enhancement and Intrinsic Force

2013

RW Clarke, A Zhukov, O Richards, N Johnson, V Ostanin, D Klenerman

J Am Chem Soc

Amyloid-β Oligomers are Sequestered by both Intracellular and Extracellular Chaperones

2012

P Narayan, S Meehan, JA Carver, MR Wilson, CM Dobson, D Klenerman

Biochemistry

A hybrid scanning mode for fast scanning ion conductance microscopy (SICM) imaging.

2012

A Zhukov, O Richards, V Ostanin, Y Korchev, D Klenerman

Ultramicroscopy

Quantitative single-molecule microscopy reveals that CENP-A(Cnp1) deposition occurs during G2 in fission yeast

2012

D Lando, U Endesfelder, H Berger, L Subramanian, PD Dunne, J McColl, D Klenerman, AM Carr, M Sauer, RC Allshire, M Heilemann, ED Laue

Open biology

Hydrodynamic trapping of molecules in lipid bilayers

2012

P Jönsson, J McColl, RW Clarke, VP Ostanin, B Jönsson, D Klenerman

Proceedings of the National Academy of Sciences of the United States of America

Direct observation of the interconversion of normal and toxic forms of α-synuclein.

2012

N Cremades, SIA Cohen, E Deas, AY Abramov, AY Chen, A Orte, M Sandal, RW Clarke, P Dunne, FA Aprile, CW Bertoncini, NW Wood, TPJ Knowles, CM Dobson, D Klenerman

Cell

Topographical and electrochemical nanoscale imaging of living cells using voltage-switching mode scanning electrochemical microscopy

2012

Y Takahashi, AI Shevchuk, P Novak, B Babakinejad, J Macpherson, PR Unwin, H Shiku, J Gorelik, D Klenerman, YE Korchev, T Matsue

Proceedings of the National Academy of Sciences

An alternative mechanism of clathrin-coated pit closure revealed by ion conductance microscopy.

2012

AI Shevchuk, P Novak, M Taylor, IA Diakonov, A Ziyadeh-Isleem, M Bitoun, P Guicheney, MJ Lab, J Gorelik, CJ Merrifield, D Klenerman, YE Korchev

The Journal of Cell Biology

The extracellular chaperone clusterin sequesters oligomeric forms of the amyloid-β 1-40 peptide

2012

P Narayan, A Orte, RW Clarke, B Bolognesi, S Hook, KA Ganzinger, S Meehan, MR Wilson, CM Dobson, D Klenerman

Nature Structural and Molecular Biology

Fluorophore conjugates for single molecule work

2012

RT Ranasinghe, D Klenerman

Ubiquitin chain conformation regulates recognition and activity of interacting proteins

2012

Y Ye, G Blaser, MH Horrocks, MJ Ruedas-Rama, S Ibrahim, AA Zhukov, A Orte, D Klenerman, SE Jackson, D Komander

Nature

Single Molecule Fluorescence under Conditions of Fast Flow

2012

MH Horrocks, H Li, J-U Shim, RT Ranasinghe, RW Clarke, WTS Huck, C Abell, D Klenerman

Analytical Chemistry

Separation and detection of bacteria using rupture event scanning

2011

MA Cooper, FN Dultsev, VP Ostanin, D Klenerman

Analytica Chimica Acta

Multifunctional Nanoprobes for Nanoscale Chemical Imaging and Localized Chemical Delivery at Surfaces and Interfaces

2011

Y Takahashi, AI Shevchuk, P Novak, Y Zhang, N Ebejer, JV Macpherson, PR Unwin, AJ Pollard, D Roy, CA Clifford, H Shiku, T Matsue, D Klenerman, YE Korchev

Angew Chem Int Ed Engl

The T cell receptor triggering apparatus is composed of monovalent or monomeric proteins

2011

JR James, J McColl, MI Oliveira, PD Dunne, E Huang, A Jansson, P Nilsson, DL Sleep, CM Gonçalves, SH Morgan, JH Felce, R Mahen, RA Fernandes, AM Carmo, D Klenerman, SJ Davis

The Journal of biological chemistry

Imaging and characterisation of the surface of live cells

2011

D Klenerman, YE Korchev, SJ Davis

Current Opinion in Chemical Biology

Realizing the biological and biomedical potential of nanoscale imaging using a pipette probe

2011

AI Shevchuk, P Novak, Y Takahashi, R Clarke, M Miragoli, B Babakinejad, J Gorelik, YE Korchev, D Klenerman

Nanomedicine (London, England)

Scanning ion conductance microscopy: a convergent high-resolution technology for multi-parametric analysis of living cardiovascular cells

2011

M Miragoli, A Moshkov, P Novak, A Shevchuk, VO Nikolaev, I El-Hamamsy, CMF Potter, P Wright, SHSA Kadir, AR Lyon, JA Mitchell, AH Chester, D Klenerman, MJ Lab, YE Korchev, SE Harding, J Gorelik

Journal of the Royal Society Interface

Multifunctional nanoprobes for nanoscale chemical imaging and localized chemical delivery at surfaces and interfaces

2011

Y Takahashi, AI Shevchuk, P Novak, Y Zhang, N Ebejer, JV MacPherson, PR Unwin, AJ Pollard, D Roy, CA Clifford, H Shiku, T Matsue, D Klenerman, YE Korchev

Angewandte Chemie - International Edition

Imaging and characterisation of the surface of live cells

2011

D Klenerman, YE Korchev, SJ Davis

Current Opinion in Chemical Biology

A chelating dendritic ligand capped quantum dot: preparation, surface passivation, bioconjugation and specific DNA detection.

2011

D Zhou, Y Li, EAH Hall, C Abell, D Klenerman

Nanoscale

Single-molecule fluorescence coincidence spectroscopy and its application to resonance energy transfer

2011

A Orte, RW Clarke, D Klenerman

ChemPhysChem

Single-molecule two-colour coincidence detection to probe biomolecular associations.

2010

A Orte, R Clarke, D Klenerman

Biochem Soc Trans

Selective Diffusion Barriers Separate Membrane Compartments

2010

A Bruckbauer, PD Dunne, P James, E Howes, D Zhou, R Jones, D Klenerman

Biophys J

Tracking diffusion of GM1 gangliosides and zona pellucida binding molecules in sperm plasma membranes following cholesterol efflux

2010

R Jones, E Howes, PD Dunne, P James, A Bruckbauer, D Klenerman

Dev Biol

RNA Conformation in Catalytically Active Human Telomerase

2010

JA Yeoman, A Orte, B Ashbridge, D Klenerman, S Balasubramanian

Journal of the American Chemical Society

Taking T cells beyond the diffraction limit.

2010

PA van der Merwe, PD Dunne, D Klenerman, SJ Davis

Nature immunology

Selective diffusion barriers separate membrane compartments

2010

A Bruckbauer, PD Dunne, P James, E Howes, D Zhou, R Jones, D Klenerman

Biophysical Journal

Nanoscale live-cell imaging using hopping probe ion conductance microscopy

2009

P Novak, C Li, AI Shevchuk, R Stepanyan, M Caldwell, S Hughes, TG Smart, J Gorelik, VP Ostanin, MJ Lab, GWJ Moss, GI Frolenkov, D Klenerman, YE Korchev

NATURE METHODS

Single-Molecule Analysis of the Human Telomerase RNA center dot Dyskerin Interaction and the Effect of Dyskeratosis Congenita Mutations

2009

B Ashbridge, A Orte, JA Yeoman, M Kirwan, T Vulliamy, I Dokal, D Klenerman, S Balasubramanian

Biochemistry

Probing neuroserpin polymerization and interaction with amyloid-beta peptides using single molecule fluorescence.

2009

A Chiou, P Hägglöf, A Orte, AY Chen, PD Dunne, D Belorgey, S Karlsson-Li, DA Lomas, D Klenerman

Biophysical Journal

DySCo: quantitating associations of membrane proteins using two-color single-molecule tracking.

2009

PD Dunne, RA Fernandes, J McColl, JW Yoon, JR James, SJ Davis, D Klenerman

Biophysical Journal

The BRC repeats of human BRCA2 differentially regulate RAD51 binding on single- versus double-stranded DNA to stimulate strand exchange

2009

MKK Shivji, SR Mukund, E Rajendra, S Chen, JM Short, J Savill, D Klenerman, AR Venkitaraman

Proceedings of the National Academy of Sciences

Nanoscale live-cell imaging using hopping probe ion conductance microscopy.

2009

P Novak, C Li, AI Shevchuk, R Stepanyan, M Caldwell, S Hughes, TG Smart, J Gorelik, VP Ostanin, MJ Lab, GWJ Moss, GI Frolenkov, D Klenerman, YE Korchev

Nat Methods

Non-invasive imaging of stem cells by scanning ion conductance microscopy: future perspective.

2008

J Gorelik, NN Ali, SH Sheikh Abdul Kadir, M Lab, P Stojkovic, L Armstrong, EV Sviderskaya, YA Negulyaev, D Klenerman, DC Bennett, M Lako, SE Harding, M Stojkovic, YE Korchev

Tissue Eng Part C Methods

Fluorescence Coincidence Spectroscopy for Single-Molecule Fluorescence Resonance Energy-Transfer Measurements

2008

A Orte, RW Clarke, D Klenerman

Analytical Chemistry

Accurate whole human genome sequencing using reversible terminator chemistry

2008

DR Bentley, S Balasubramanian, HP Swerdlow, GP Smith, J Milton, CG Brown, KP Hall, DJ Evers, CL Barnes, HR Bignell, JM Boutell, J Bryant, RJ Carter, R Keira Cheetham, AJ Cox, DJ Ellis, MR Flatbush, NA Gormley, SJ Humphray, LJ Irving, MS Karbelashvili, SM Kirk, H Li, X Liu, KS Maisinger, LJ Murray, B Obradovic, T Ost, ML Parkinson, MR Pratt, IMJ Rasolonjatovo, MT Reed, R Rigatti, C Rodighiero, MT Ross, A Sabot, SV Sankar, A Scally, GP Schroth, ME Smith, VP Smith, A Spiridou, PE Torrance, SS Tzonev, EH Vermaas, K Walter, X Wu, L Zhang, MD Alam, C Anastasi, IC Aniebo, DMD Bailey, IR Bancarz, S Banerjee, SG Barbour, PA Baybayan, VA Benoit, KF Benson, C Bevis, PJ Black, A Boodhun, JS Brennan, JA Bridgham, RC Brown, AA Brown, DH Buermann, AA Bundu, JC Burrows, NP Carter, N Castillo, M Chiara E Catenazzi, S Chang, R Neil Cooley, NR Crake, OO Dada, KD Diakoumakos, B Dominguez-Fernandez, DJ Earnshaw, UC Egbujor, DW Elmore, SS Etchin, MR Ewan, M Fedurco, LJ Fraser, KV Fuentes Fajardo, W Scott Furey, D George, KJ Gietzen, CP Goddard, GS Golda, PA Granieri, DE Green, DL Gustafson, NF Hansen, K Harnish, CD Haudenschild, NI Heyer, MM Hims, JT Ho, AM Horgan, K Hoschler, S Hurwitz, DV Ivanov, MQ Johnson, T James, TA Huw Jones, G-D Kang, TH Kerelska, AD Kersey, I Khrebtukova, AP Kindwall, Z Kingsbury, PI Kokko-Gonzales, A Kumar, MA Laurent, CT Lawley, SE Lee, X Lee, AK Liao, JA Loch, M Lok, S Luo, RM Mammen, JW Martin, PG McCauley, P McNitt, P Mehta, KW Moon, JW Mullens, T Newington, Z Ning, B Ling Ng, SM Novo, MJ O'Neill, MA Osborne, A Osnowski, O Ostadan, LL Paraschos, L Pickering, AC Pike, AC Pike, D Chris Pinkard, DP Pliskin, J Podhasky, VJ Quijano, C Raczy, VH Rae, SR Rawlings, A Chiva Rodriguez, PM Roe, J Rogers, MC Rogert Bacigalupo, N Romanov, A Romieu, RK Roth, NJ Rourke, ST Ruediger, E Rusman, RM Sanches-Kuiper, MR Schenker, JM Seoane, RJ Shaw, MK Shiver, SW Short, NL Sizto, JP Sluis, MA Smith, J Ernest Sohna Sohna, EJ Spence, K Stevens, N Sutton, L Szajkowski, CL Tregidgo, G Turcatti, S Vandevondele, Y Verhovsky, SM Virk, S Wakelin, GC Walcott, J Wang, GJ Worsley, J Yan, L Yau, M Zuerlein, J Rogers, JC Mullikin, ME Hurles, NJ McCooke, JS West, FL Oaks, PL Lundberg, D Klenerman, R Durbin, AJ Smith

Nature

Direct characterization of amyloidogenic oligomers by single-molecule fluorescence

2008

A Orte, NR Birkett, RW Clarke, GL Devlin, CM Dobson, D Klenerman

Proceedings of the National Academy of Sciences of the United States of America

Noncontact measurement of the local mechanical properties of living cells using pressure applied via a pipette

2008

D Sánchez, N Johnson, C Li, P Novak, J Rheinlaender, Y Zhang, U Anand, P Anand, J Gorelik, GI Frolenkov, C Benham, M Lab, VP Ostanin, TE Schäffer, D Klenerman, YE Korchev

Biophysical journal

Characterization and application of controllable local chemical changes produced by reagent delivery from a nanopipet.

2008

JD Piper, C Li, C-J Lo, R Berry, Y Korchev, L Ying, D Klenerman

J Am Chem Soc

High resolution imaging using scanning ion conductance microscopy with improved distance feedback control

2008

C Li, N Johnson, V Ostanin, A Shevchuk, LM Ying, Y Korchev, D Klenerman

Progress in Natural Science

Bayesian inference for improved single molecule fluorescence tracking

2008

JW Yoon, A Bruckbauer, WJ Fitzgerald, D Klenerman

Biophys J

Imaging single virus particles on the surface of cell membranes by high-resolution scanning surface confocal microscopy.

2008

AI Shevchuk, P Hobson, MJ Lab, D Klenerman, N Krauzewicz, YE Korchev

Biophysical journal

Single-molecule analysis of human telomerase monomer.

2008

D Alves, H Li, R Codrington, A Orte, X Ren, D Klenerman, S Balasubramanian

Nature chemical biology

Endocytic pathways: combined scanning ion conductance and surface confocal microscopy study

2008

AI Shevchuk, P Hobson, MJ Lab, D Klenerman, N Krauzewicz, YE Korchev

Pflugers Arch

A Compact Functional Quantum Dot−DNA Conjugate:  Preparation, Hybridization, and Specific Label-Free DNA Detection

2008

D Zhou, L Ying, X Hong, EA Hall, C Abell, D Klenerman

Langmuir : the ACS journal of surfaces and colloids

Noncontact measurement of the local mechanical properties of living cells using pressure applied via a pipette

2008

D Sánchez, N Johnson, C Li, P Novak, J Rheinlaender, Y Zhang, U Anand, P Anand, J Gorelik, GI Frolenkov, C Benham, M Lab, VP Ostanin, TE Schäffer, D Klenerman, YE Korchev

Biophysical Journal

Evidence of an Intermediate and Parallel Pathways in Protein Unfolding from Single-Molecule Fluorescence

2008

A Orte, TD Craggs, SS White, SE Jackson, D Klenerman

Journal of the American Chemical Society

Single-molecule level analysis of the subunit composition of the T cell receptor on live T cells

2007

JR James, SS White, RW Clarke, AM Johansen, PD Dunne, DL Sleep, WJ Fitzgerald, SJ Davis, D Klenerman

Proceedings of the National Academy of Sciences

Nanopipette delivery of individual molecules to cellular compartments for single-molecule fluorescence tracking.

2007

A Bruckbauer, P James, D Zhou, JW Yoon, D Excell, Y Korchev, R Jones, D Klenerman

Biophysical journal

Two-color fluorescence analysis of individual virions determines the distribution of the copy number of proteins in herpes simplex virus particles.

2007

RW Clarke, N Monnier, H Li, D Zhou, H Browne, D Klenerman

Biophysical Journal

Supramolecular organization of the sperm plasma membrane during maturation and capacitation

2007

R Jones, PS James, L Howes, A Bruckbauer, D Klenerman

Asian J Androl

Basolateral P2X4-like receptors regulate the extracellular ATP-stimulated epithelial Na+ channel activity in renal epithelia.

2007

Y Zhang, D Sanchez, J Gorelik, D Klenerman, M Lab, C Edwards, Y Korchev

Am J Physiol Renal Physiol

Surface Conductivity of Biological Macromolecules Measured by Nanopipette Dielectrophoresis

2007

RW Clarke, JD Piper, L Ying, D Klenerman

Phys Rev Lett

Optimized threshold selection for single-molecule two-color fluorescence coincidence spectroscopy.

2007

RW Clarke, A Orte, D Klenerman

Anal Chem

Localized and non-contact mechanical stimulation of dorsal root ganglion sensory neurons using scanning ion conductance microscopy.

2007

D Sánchez, U Anand, J Gorelik, CD Benham, C Bountra, M Lab, D Klenerman, R Birch, P Anand, Y Korchev

Journal of Neuroscience Methods

YFP unfolding kinetics studied in a single-molecule nano-flow cell

2007

A Orte, TD Craggs, SS White, SE Jackson, D Klenerman

BIOPHYSICAL JOURNAL

Improved scanning ion conductance microscopy (SICM) for live cell imaging

2007

N Johnson, C Li, V Ostanin, A Bruckbauer, Y Korchev, D Klenerman

BIOPHYSICAL JOURNAL

Single molecule studies of the stoichiometry of human telomerase complex

2007

R Codrington, D Alves, H Li, A Orte, XJ Ren, LM Ying, D Klenerman, S Balasubramanian

BIOPHYSICAL JOURNAL

Basolateral P2X<inf>4</inf>-like receptors regulate the extracellular ATP-stimulated epithelial Na<sup>+</sup> channel activity in renal epithelia

2007

Y Zhang, D Sanchez, J Gorelik, D Klenerman, M Lab, C Edwards, Y Korchev

American Journal of Physiology - Renal Physiology

A Renewable Nanosensor Based on a Glass Nanopipette

2006

JD Piper, RW Clarke, YE Korchev, L Ying, D Klenerman

J Am Chem Soc

A Simple Nanomixer for Single-Molecule Kinetics Measurements

2006

SS White, S Balasubramanian, D Klenerman, L Ying

Angewandte Chemie

Determination of the Fraction and Stoichiometry of Femtomolar Levels of Biomolecular Complexes in an Excess of Monomer Using Single-Molecule, Two-Color Coincidence Detection

2006

A Orte, R Clarke, S Balasubramanian, D Klenerman

Analytical chemistry

Structural analysis of the catalytic core of human telomerase RNA by FRET and molecular modeling

2006

G Gavory, MF Symmons, Y Krishnan Ghosh, D Klenerman, S Balasubramanian

Biochemistry

Characterization of a single molecule DNA switch in free solution.

2006

SS White, H Li, RJ Marsh, JD Piper, ND Leonczek, N Nicolaou, AJ Bain, L Ying, D Klenerman

J Am Chem Soc

Investigating a Quadruplex−Ligand Interaction by Unfolding Kinetics

2006

JJ Green, S Ladame, L Ying, D Klenerman, S Balasubramanian

J Am Chem Soc

Potential biomedical applications of the scanned nanopipette.

2006

D Klenerman, Y Korchev

Nanomedicine (Lond)

Evidence for resonance optical trapping of individual fluorophore-labeled antibodies using single molecule fluorescence spectroscopy.

2006

H Li, D Zhou, H Browne, D Klenerman

Journal of the American Chemical Society

Building three-dimensional nanostructures with active enzymes by surface templated layer-by-layer assembly.

2006

S Rauf, D Zhou, C Abell, D Klenerman, D-J Kang

Chemical communications (Cambridge, England)

Analysis of human telomerase activity and function by two color single molecule coincidence fluorescence spectroscopy.

2006

X Ren, H Li, RW Clarke, DA Alves, L Ying, D Klenerman, S Balasubramanian

Journal of the American Chemical Society

Imaging Proteins in Membranes of Living Cells by High-Resolution Scanning Ion Conductance Microscopy

2006

AI Shevchuk, GI Frolenkov, D Sánchez, PS James, N Freedman, MJ Lab, R Jones, D Klenerman, YE Korchev

Angewandte Chemie (International ed. in English)

Macroscopic 2D networks self-assembled from nanometer-sized protein/DNA complexes.

2006

M Manzanera, DJ Frankel, H Li, D Zhou, A Bruckbauer, P Kreutzmann, JM Blackburn, C Abell, T Rayment, D Klenerman, PD Barker

Nano letters

A reversible pH-driven DNA nanoswitch array.

2006

D Liu, A Bruckbauer, C Abell, S Balasubramanian, D-J Kang, D Klenerman, D Zhou

Journal of the American Chemical Society

Nanoscale pipetting for controlled chemistry in small arrayed water droplets using a double-barrel pipet

2006

KT Rodolfa, A Bruckbauer, D Zhou, AI Schevchuk, YE Korchev, D Klenerman

Nano Letters

A simple nanomixer for single-molecule kinetics measurements.

2006

SS White, S Balasubramanian, D Klenerman, L Ying

Angewandte Chemie - International Edition

Two-component graded deposition of biomolecules with a double-barreled nanopipette.

2005

KT Rodolfa, A Bruckbauer, D Zhou, YE Korchev, D Klenerman

Angew Chem Int Ed Engl

Two-Component Graded Deposition of Biomolecules with a Double-Barreled Nanopipette

2005

KT Rodolfa, A Bruckbauer, D Zhou, YE Korchev, D Klenerman

Angewandte Chemie

Aldosterone acts via an ATP autocrine/paracrine system: the Edelman ATP hypothesis revisited.

2005

J Gorelik, Y Zhang, D Sánchez, A Shevchuk, G Frolenkov, M Lab, D Klenerman, C Edwards, Y Korchev

Proceedings of the National Academy of Sciences of the United States of America

Fluorescence resonance energy transfer between a quantum dot donor and a dye acceptor attached to DNA

2005

D Zhou, JD Piper, C Abell, D Klenerman, D-J Kang, L Ying

Chem Commun (Camb)

Scanning ion conductance microscopy reveals how a functional renal epithelial monolayer maintains its integrity

2005

Y Zhang, J Gorelik, D Sanchez, A Shevchuk, M Lab, I Vodyanoy, D Klenerman, C Edwards, Y Korchev

Kidney Int

The scanned nanopipette: A new tool for high resolution bioimaging and controlled deposition of biomolecules

2005

L Ying, A Bruckbauer, D Zhou, J Gorelik, A Shevchuk, M Lab, Y Korchev, D Klenerman

Physical Chemistry Chemical Physics

Trapping of Proteins under Physiological Conditions in a Nanopipette

2005

RW Clarke, SS White, D Zhou, L Ying, D Klenerman

Angew Chem Int Ed Engl

Fabrication of three-dimensional surface structures with highly fluorescent quantum dots by surface-templated layer-by-layer assembly

2005

DJ Zhou, A Bruckbauer, C Abell, D Klenerman, DJ Kang

Advanced Materials

Aldosterone acts via an ATP autocrine/paracrine system: the Edelman ATP hypothesis revisited.

2005

J Gorelik, Y Zhang, D Sánchez, A Shevchuk, G Frolenkov, M Lab, D Klenerman, C Edwards, Y Korchev

Proceedings of the National Academy of Sciences of the United States of America