Publications

Analysis of α-synuclein species enriched from cerebral cortex of humans with sporadic dementia with Lewy bodies.

2020

Sanderson JB, De S, Jiang H, Rovere M, Jin M, Zaccagnini L, Hays Watson A, De Boni L, Lagomarsino VN, Young-Pearse TL, Liu X, Pochapsky TC, Hyman BT, Dickson DW, Klenerman D, Selkoe DJ, Bartels T.

Brain Commun.

Digital Sensing and Molecular Computation by an Enzyme-Free DNA Circuit.

2020

Arter WE, Yusim Y, Peter Q, Taylor CG, Klenerman D, Keyser UF, Knowles TPJ

ACS Nano.

The molecular processes underpinning prion-like spreading and seed amplification in protein aggregation.

2020

Meisl G, Knowles TP, Klenerman D.

Curr Opin Neurobiol.

The Costs of Close Contacts: Visualizing the Energy Landscape of Cell Contacts at the Nanoscale.

2020

Kulenkampff K, Lippert AH, McColl J, Santos AM, Ponjavic A, Jenkins E, Humphrey J, Winkel A, Franze K, Lee SF, Davis SJ, Klenerman D.

Biophys J.

Beta amyloid aggregates induce sensitised TLR4 signalling causing long-term potentiation deficit and rat neuronal cell death.

2020

Hughes C, Choi ML, Yi JH, Kim SC, Drews A, George-Hyslop PS, Bryant C, Gandhi S, Cho K, Klenerman D.

Commun Biol.

MyD88 Death-Domain Oligomerization Determines Myddosome Architecture: Implications for Toll-like Receptor Signaling.

2020

Moncrieffe MC, Bollschweiler D, Li B, Penczek PA, Hopkins L, Bryant CE, Klenerman D, Gay NJ.

Structure

A General in Vitro Assay for Studying Enzymatic Activities of the Ubiquitin System.

2020

Zuo Y, Chong BK, Jiang K, Finley D, Klenerman D, Ye Y.

Biochemistry

α-Synuclein strains target distinct brain regions and cell types.

2020

Lau A, So RWL, Lau HHC, Sang JC, Ruiz-Riquelme A, Fleck SC, Stuart E, Menon S, Visanji NP, Meisl G, Faidi R, Marano MM, Schmitt-Ulms C, Wang Z, Fraser PE, Tandon A, Hyman BT, Wille H, Ingelsson M, Klenerman D, Watts JC.

Nat Neurosci.

N-Terminal Ubiquitination of Amyloidogenic Proteins Triggers Removal of Their Oligomers by the Proteasome Holoenzyme

2020

Ye Y, Klenerman D, Finley D.

J Mol Biol.

Nanoscale-targeted patch-clamp recordings of functional presynaptic ion channels.

2013

P Novak, J Gorelik, U Vivekananda, AI Shevchuk, YS Ermolyuk, RJ Bailey, AJ Bushby, GWJ Moss, DA Rusakov, D Klenerman, DM Kullmann, KE Volynski, YE Korchev

Neuron

Local delivery of molecules from a nanopipette for quantitative receptor mapping on live cells

2013

B Babakinejad, P Jönsson, A López Córdoba, P Actis, P Novak, Y Takahashi, A Shevchuk, U Anand, P Anand, A Drews, A Ferrer-Montiel, D Klenerman, YE Korchev

Analytical Chemistry

A Single gD Glycoprotein Can Mediate Infection by Herpes simplex Virus

2013

RW Clarke, A Drews, H Browne, D Klenerman

Journal of the American Chemical Society

Ultrarapid Generation of Femtoliter Microfluidic Droplets for Single-Molecule-Counting Immunoassays

2013

J-U Shim, RT Ranasinghe, CA Smith, SM Ibrahim, F Hollfelder, WTS Huck, D Klenerman, C Abell

ACS Nano

A Single Molecule Study of Toll-Like-Receptor 4 Structure and Signalling

2013

SL Latty, K Ganzinger, LJ Hopkins, C Bryant, D Klenerman

EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS

Single molecule studies of protein aggregates

2013

D Klenerman

EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS

Using single molecule techniques to find out how the major protein associated with Parkinson's disease

2013

MH Horrocks, L Tosatto, N Cremades, CM Dobson, D Klenerman

EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS

Molecular nanomechanics and local stimulus of individual biomolecules on the surface of cells

2013

P Joensson, AL Cordoba, B Babakinejad, A Drews, SJ Davis, YE Korchev, D Klenerman

EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS

Single molecule fluorescence approach to investigate alpha-synuclein oligomer formation

2013

L Tosatto, MH Horrocks, N Cremades, T Guilliams, M Dalla Serra, D Klenerman

EUROPEAN BIOPHYSICS JOURNAL WITH BIOPHYSICS LETTERS

Single-molecule measurements of transient biomolecular complexes through microfluidic dilution

2013

MH Horrocks, L Rajah, P Jönsson, M Kjaergaard, M Vendruscolo, TPJ Knowles, D Klenerman

Analytical chemistry

Highly Rapid Amplification-Free and Quantitative DNA Imaging Assay

2013

T Klamp, M Camps, B Nieto, F Guasch, RT Ranasinghe, J Wiedemann, Z Petrášek, P Schwille, D Klenerman, M Sauer

Scientific reports

Three-Dimensional RNA Structure of the Major HIV-1 Packaging Signal Region

2013

JD Stephenson, H Li, JC Kenyon, M Symmons, D Klenerman, AML Lever

Structure

Single molecule characterization of the interactions between amyloid-β peptides and the membranes of hippocampal cells

2013

P Narayan, KA Ganzinger, J McColl, L Weimann, S Meehan, S Qamar, JA Carver, MR Wilson, P St George-Hyslop, CM Dobson, D Klenerman

J Am Chem Soc

Imaging the cell surface and its organization down to the level of single molecules.

2013

D Klenerman, A Shevchuk, P Novak, YE Korchev, SJ Davis

Philosophical transactions of the Royal Society of London. Series B, Biological sciences

A quantitative comparison of single-dye tracking analysis tools using Monte Carlo simulations.

2013

L Weimann, KA Ganzinger, J McColl, KL Irvine, SJ Davis, NJ Gay, CE Bryant, D Klenerman

PloS one

Imaging the cell surface and its organization down to the level of single molecules

2013

D Klenerman, A Shevchuk, P Novak, YE Korchev, SJ Davis

Philosophical Transactions of the Royal Society B: Biological Sciences

Megahertz-generated femtoliter microfluidic droplets for single-molecule-counting immunoassay

2013

JU Shim, RT Ranasinghe, F Hollfelder, WTS Huck, D Klenerman, C Abell, J Cooper

17th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2013

Nanoscale-targeted patch-clamp recordings of functional presynaptic ion channels.

2013

P Novak, J Gorelik, U Vivekananda, AI Shevchuk, YS Ermolyuk, RJ Bailey, AJ Bushby, GWJ Moss, DA Rusakov, D Klenerman, DM Kullmann, KE Volynski, YE Korchev

Neuron

Three-dimensional RNA structure of the major HIV-1 packaging signal region

2013

JD Stephenson, H Li, JC Kenyon, M Symmons, D Klenerman, AML Lever

Structure

Ubiquitin chain conformation regulates recognition and activity of interacting proteins

2012

Y Ye, G Blaser, MH Horrocks, MJ Ruedas-Rama, S Ibrahim, AA Zhukov, A Orte, D Klenerman, SE Jackson, D Komander

Nature

Pipette–Surface Interaction: Current Enhancement and Intrinsic Force

2013

RW Clarke, A Zhukov, O Richards, N Johnson, V Ostanin, D Klenerman

J Am Chem Soc

Amyloid-β Oligomers are Sequestered by both Intracellular and Extracellular Chaperones

2012

P Narayan, S Meehan, JA Carver, MR Wilson, CM Dobson, D Klenerman

Biochemistry

A hybrid scanning mode for fast scanning ion conductance microscopy (SICM) imaging.

2012

A Zhukov, O Richards, V Ostanin, Y Korchev, D Klenerman

Ultramicroscopy

Quantitative single-molecule microscopy reveals that CENP-A(Cnp1) deposition occurs during G2 in fission yeast

2012

D Lando, U Endesfelder, H Berger, L Subramanian, PD Dunne, J McColl, D Klenerman, AM Carr, M Sauer, RC Allshire, M Heilemann, ED Laue

Open biology

Hydrodynamic trapping of molecules in lipid bilayers

2012

P Jönsson, J McColl, RW Clarke, VP Ostanin, B Jönsson, D Klenerman

Proceedings of the National Academy of Sciences of the United States of America

Direct observation of the interconversion of normal and toxic forms of α-synuclein.

2012

N Cremades, SIA Cohen, E Deas, AY Abramov, AY Chen, A Orte, M Sandal, RW Clarke, P Dunne, FA Aprile, CW Bertoncini, NW Wood, TPJ Knowles, CM Dobson, D Klenerman

Cell

Topographical and electrochemical nanoscale imaging of living cells using voltage-switching mode scanning electrochemical microscopy

2012

Y Takahashi, AI Shevchuk, P Novak, B Babakinejad, J Macpherson, PR Unwin, H Shiku, J Gorelik, D Klenerman, YE Korchev, T Matsue

Proceedings of the National Academy of Sciences

An alternative mechanism of clathrin-coated pit closure revealed by ion conductance microscopy.

2012

AI Shevchuk, P Novak, M Taylor, IA Diakonov, A Ziyadeh-Isleem, M Bitoun, P Guicheney, MJ Lab, J Gorelik, CJ Merrifield, D Klenerman, YE Korchev

The Journal of Cell Biology

The extracellular chaperone clusterin sequesters oligomeric forms of the amyloid-β 1-40 peptide

2012

P Narayan, A Orte, RW Clarke, B Bolognesi, S Hook, KA Ganzinger, S Meehan, MR Wilson, CM Dobson, D Klenerman

Nature Structural and Molecular Biology

Fluorophore conjugates for single molecule work

2012

RT Ranasinghe, D Klenerman

Ubiquitin chain conformation regulates recognition and activity of interacting proteins

2012

Y Ye, G Blaser, MH Horrocks, MJ Ruedas-Rama, S Ibrahim, AA Zhukov, A Orte, D Klenerman, SE Jackson, D Komander

Nature

Single Molecule Fluorescence under Conditions of Fast Flow

2012

MH Horrocks, H Li, J-U Shim, RT Ranasinghe, RW Clarke, WTS Huck, C Abell, D Klenerman

Analytical Chemistry

Separation and detection of bacteria using rupture event scanning

2011

MA Cooper, FN Dultsev, VP Ostanin, D Klenerman

Analytica Chimica Acta

Multifunctional Nanoprobes for Nanoscale Chemical Imaging and Localized Chemical Delivery at Surfaces and Interfaces

2011

Y Takahashi, AI Shevchuk, P Novak, Y Zhang, N Ebejer, JV Macpherson, PR Unwin, AJ Pollard, D Roy, CA Clifford, H Shiku, T Matsue, D Klenerman, YE Korchev

Angew Chem Int Ed Engl

The T cell receptor triggering apparatus is composed of monovalent or monomeric proteins

2011

JR James, J McColl, MI Oliveira, PD Dunne, E Huang, A Jansson, P Nilsson, DL Sleep, CM Gonçalves, SH Morgan, JH Felce, R Mahen, RA Fernandes, AM Carmo, D Klenerman, SJ Davis

The Journal of biological chemistry

Imaging and characterisation of the surface of live cells

2011

D Klenerman, YE Korchev, SJ Davis

Current Opinion in Chemical Biology

Realizing the biological and biomedical potential of nanoscale imaging using a pipette probe

2011

AI Shevchuk, P Novak, Y Takahashi, R Clarke, M Miragoli, B Babakinejad, J Gorelik, YE Korchev, D Klenerman

Nanomedicine (London, England)

Scanning ion conductance microscopy: a convergent high-resolution technology for multi-parametric analysis of living cardiovascular cells

2011

M Miragoli, A Moshkov, P Novak, A Shevchuk, VO Nikolaev, I El-Hamamsy, CMF Potter, P Wright, SHSA Kadir, AR Lyon, JA Mitchell, AH Chester, D Klenerman, MJ Lab, YE Korchev, SE Harding, J Gorelik

Journal of the Royal Society Interface

Multifunctional nanoprobes for nanoscale chemical imaging and localized chemical delivery at surfaces and interfaces

2011

Y Takahashi, AI Shevchuk, P Novak, Y Zhang, N Ebejer, JV MacPherson, PR Unwin, AJ Pollard, D Roy, CA Clifford, H Shiku, T Matsue, D Klenerman, YE Korchev

Angewandte Chemie - International Edition

Imaging and characterisation of the surface of live cells

2011

D Klenerman, YE Korchev, SJ Davis

Current Opinion in Chemical Biology

A chelating dendritic ligand capped quantum dot: preparation, surface passivation, bioconjugation and specific DNA detection.

2011

D Zhou, Y Li, EAH Hall, C Abell, D Klenerman

Nanoscale

Single-molecule fluorescence coincidence spectroscopy and its application to resonance energy transfer

2011

A Orte, RW Clarke, D Klenerman

ChemPhysChem

Single-molecule two-colour coincidence detection to probe biomolecular associations.

2010

A Orte, R Clarke, D Klenerman

Biochem Soc Trans

Selective Diffusion Barriers Separate Membrane Compartments

2010

A Bruckbauer, PD Dunne, P James, E Howes, D Zhou, R Jones, D Klenerman

Biophys J

Tracking diffusion of GM1 gangliosides and zona pellucida binding molecules in sperm plasma membranes following cholesterol efflux

2010

R Jones, E Howes, PD Dunne, P James, A Bruckbauer, D Klenerman

Dev Biol

RNA Conformation in Catalytically Active Human Telomerase

2010

JA Yeoman, A Orte, B Ashbridge, D Klenerman, S Balasubramanian

Journal of the American Chemical Society

Taking T cells beyond the diffraction limit.

2010

PA van der Merwe, PD Dunne, D Klenerman, SJ Davis

Nature immunology

Selective diffusion barriers separate membrane compartments

2010

A Bruckbauer, PD Dunne, P James, E Howes, D Zhou, R Jones, D Klenerman

Biophysical Journal

Nanoscale live-cell imaging using hopping probe ion conductance microscopy

2009

P Novak, C Li, AI Shevchuk, R Stepanyan, M Caldwell, S Hughes, TG Smart, J Gorelik, VP Ostanin, MJ Lab, GWJ Moss, GI Frolenkov, D Klenerman, YE Korchev

NATURE METHODS

Single-Molecule Analysis of the Human Telomerase RNA center dot Dyskerin Interaction and the Effect of Dyskeratosis Congenita Mutations

2009

B Ashbridge, A Orte, JA Yeoman, M Kirwan, T Vulliamy, I Dokal, D Klenerman, S Balasubramanian

Biochemistry

Probing neuroserpin polymerization and interaction with amyloid-beta peptides using single molecule fluorescence.

2009

A Chiou, P Hägglöf, A Orte, AY Chen, PD Dunne, D Belorgey, S Karlsson-Li, DA Lomas, D Klenerman

Biophysical Journal

DySCo: quantitating associations of membrane proteins using two-color single-molecule tracking.

2009

PD Dunne, RA Fernandes, J McColl, JW Yoon, JR James, SJ Davis, D Klenerman

Biophysical Journal

The BRC repeats of human BRCA2 differentially regulate RAD51 binding on single- versus double-stranded DNA to stimulate strand exchange

2009

MKK Shivji, SR Mukund, E Rajendra, S Chen, JM Short, J Savill, D Klenerman, AR Venkitaraman

Proceedings of the National Academy of Sciences

Nanoscale live-cell imaging using hopping probe ion conductance microscopy.

2009

P Novak, C Li, AI Shevchuk, R Stepanyan, M Caldwell, S Hughes, TG Smart, J Gorelik, VP Ostanin, MJ Lab, GWJ Moss, GI Frolenkov, D Klenerman, YE Korchev

Nat Methods

Non-invasive imaging of stem cells by scanning ion conductance microscopy: future perspective.

2008

J Gorelik, NN Ali, SH Sheikh Abdul Kadir, M Lab, P Stojkovic, L Armstrong, EV Sviderskaya, YA Negulyaev, D Klenerman, DC Bennett, M Lako, SE Harding, M Stojkovic, YE Korchev

Tissue Eng Part C Methods

Fluorescence Coincidence Spectroscopy for Single-Molecule Fluorescence Resonance Energy-Transfer Measurements

2008

A Orte, RW Clarke, D Klenerman

Analytical Chemistry

Accurate whole human genome sequencing using reversible terminator chemistry

2008

DR Bentley, S Balasubramanian, HP Swerdlow, GP Smith, J Milton, CG Brown, KP Hall, DJ Evers, CL Barnes, HR Bignell, JM Boutell, J Bryant, RJ Carter, R Keira Cheetham, AJ Cox, DJ Ellis, MR Flatbush, NA Gormley, SJ Humphray, LJ Irving, MS Karbelashvili, SM Kirk, H Li, X Liu, KS Maisinger, LJ Murray, B Obradovic, T Ost, ML Parkinson, MR Pratt, IMJ Rasolonjatovo, MT Reed, R Rigatti, C Rodighiero, MT Ross, A Sabot, SV Sankar, A Scally, GP Schroth, ME Smith, VP Smith, A Spiridou, PE Torrance, SS Tzonev, EH Vermaas, K Walter, X Wu, L Zhang, MD Alam, C Anastasi, IC Aniebo, DMD Bailey, IR Bancarz, S Banerjee, SG Barbour, PA Baybayan, VA Benoit, KF Benson, C Bevis, PJ Black, A Boodhun, JS Brennan, JA Bridgham, RC Brown, AA Brown, DH Buermann, AA Bundu, JC Burrows, NP Carter, N Castillo, M Chiara E Catenazzi, S Chang, R Neil Cooley, NR Crake, OO Dada, KD Diakoumakos, B Dominguez-Fernandez, DJ Earnshaw, UC Egbujor, DW Elmore, SS Etchin, MR Ewan, M Fedurco, LJ Fraser, KV Fuentes Fajardo, W Scott Furey, D George, KJ Gietzen, CP Goddard, GS Golda, PA Granieri, DE Green, DL Gustafson, NF Hansen, K Harnish, CD Haudenschild, NI Heyer, MM Hims, JT Ho, AM Horgan, K Hoschler, S Hurwitz, DV Ivanov, MQ Johnson, T James, TA Huw Jones, G-D Kang, TH Kerelska, AD Kersey, I Khrebtukova, AP Kindwall, Z Kingsbury, PI Kokko-Gonzales, A Kumar, MA Laurent, CT Lawley, SE Lee, X Lee, AK Liao, JA Loch, M Lok, S Luo, RM Mammen, JW Martin, PG McCauley, P McNitt, P Mehta, KW Moon, JW Mullens, T Newington, Z Ning, B Ling Ng, SM Novo, MJ O'Neill, MA Osborne, A Osnowski, O Ostadan, LL Paraschos, L Pickering, AC Pike, AC Pike, D Chris Pinkard, DP Pliskin, J Podhasky, VJ Quijano, C Raczy, VH Rae, SR Rawlings, A Chiva Rodriguez, PM Roe, J Rogers, MC Rogert Bacigalupo, N Romanov, A Romieu, RK Roth, NJ Rourke, ST Ruediger, E Rusman, RM Sanches-Kuiper, MR Schenker, JM Seoane, RJ Shaw, MK Shiver, SW Short, NL Sizto, JP Sluis, MA Smith, J Ernest Sohna Sohna, EJ Spence, K Stevens, N Sutton, L Szajkowski, CL Tregidgo, G Turcatti, S Vandevondele, Y Verhovsky, SM Virk, S Wakelin, GC Walcott, J Wang, GJ Worsley, J Yan, L Yau, M Zuerlein, J Rogers, JC Mullikin, ME Hurles, NJ McCooke, JS West, FL Oaks, PL Lundberg, D Klenerman, R Durbin, AJ Smith

Nature

Direct characterization of amyloidogenic oligomers by single-molecule fluorescence

2008

A Orte, NR Birkett, RW Clarke, GL Devlin, CM Dobson, D Klenerman

Proceedings of the National Academy of Sciences of the United States of America

Noncontact measurement of the local mechanical properties of living cells using pressure applied via a pipette

2008

D Sánchez, N Johnson, C Li, P Novak, J Rheinlaender, Y Zhang, U Anand, P Anand, J Gorelik, GI Frolenkov, C Benham, M Lab, VP Ostanin, TE Schäffer, D Klenerman, YE Korchev

Biophysical journal

Characterization and application of controllable local chemical changes produced by reagent delivery from a nanopipet.

2008

JD Piper, C Li, C-J Lo, R Berry, Y Korchev, L Ying, D Klenerman

J Am Chem Soc

High resolution imaging using scanning ion conductance microscopy with improved distance feedback control

2008

C Li, N Johnson, V Ostanin, A Shevchuk, LM Ying, Y Korchev, D Klenerman

Progress in Natural Science

Bayesian inference for improved single molecule fluorescence tracking

2008

JW Yoon, A Bruckbauer, WJ Fitzgerald, D Klenerman

Biophys J

Imaging single virus particles on the surface of cell membranes by high-resolution scanning surface confocal microscopy.

2008

AI Shevchuk, P Hobson, MJ Lab, D Klenerman, N Krauzewicz, YE Korchev

Biophysical journal

Single-molecule analysis of human telomerase monomer.

2008

D Alves, H Li, R Codrington, A Orte, X Ren, D Klenerman, S Balasubramanian

Nature chemical biology

Endocytic pathways: combined scanning ion conductance and surface confocal microscopy study

2008

AI Shevchuk, P Hobson, MJ Lab, D Klenerman, N Krauzewicz, YE Korchev

Pflugers Arch

A Compact Functional Quantum Dot−DNA Conjugate:  Preparation, Hybridization, and Specific Label-Free DNA Detection

2008

D Zhou, L Ying, X Hong, EA Hall, C Abell, D Klenerman

Langmuir : the ACS journal of surfaces and colloids

Noncontact measurement of the local mechanical properties of living cells using pressure applied via a pipette

2008

D Sánchez, N Johnson, C Li, P Novak, J Rheinlaender, Y Zhang, U Anand, P Anand, J Gorelik, GI Frolenkov, C Benham, M Lab, VP Ostanin, TE Schäffer, D Klenerman, YE Korchev

Biophysical Journal

Evidence of an Intermediate and Parallel Pathways in Protein Unfolding from Single-Molecule Fluorescence

2008

A Orte, TD Craggs, SS White, SE Jackson, D Klenerman

Journal of the American Chemical Society

Single-molecule level analysis of the subunit composition of the T cell receptor on live T cells

2007

JR James, SS White, RW Clarke, AM Johansen, PD Dunne, DL Sleep, WJ Fitzgerald, SJ Davis, D Klenerman

Proceedings of the National Academy of Sciences

Nanopipette delivery of individual molecules to cellular compartments for single-molecule fluorescence tracking.

2007

A Bruckbauer, P James, D Zhou, JW Yoon, D Excell, Y Korchev, R Jones, D Klenerman

Biophysical journal

Two-color fluorescence analysis of individual virions determines the distribution of the copy number of proteins in herpes simplex virus particles.

2007

RW Clarke, N Monnier, H Li, D Zhou, H Browne, D Klenerman

Biophysical Journal

Supramolecular organization of the sperm plasma membrane during maturation and capacitation

2007

R Jones, PS James, L Howes, A Bruckbauer, D Klenerman

Asian J Androl

Basolateral P2X4-like receptors regulate the extracellular ATP-stimulated epithelial Na+ channel activity in renal epithelia.

2007

Y Zhang, D Sanchez, J Gorelik, D Klenerman, M Lab, C Edwards, Y Korchev

Am J Physiol Renal Physiol

Surface Conductivity of Biological Macromolecules Measured by Nanopipette Dielectrophoresis

2007

RW Clarke, JD Piper, L Ying, D Klenerman

Phys Rev Lett

Optimized threshold selection for single-molecule two-color fluorescence coincidence spectroscopy.

2007

RW Clarke, A Orte, D Klenerman

Anal Chem

Localized and non-contact mechanical stimulation of dorsal root ganglion sensory neurons using scanning ion conductance microscopy.

2007

D Sánchez, U Anand, J Gorelik, CD Benham, C Bountra, M Lab, D Klenerman, R Birch, P Anand, Y Korchev

Journal of Neuroscience Methods

YFP unfolding kinetics studied in a single-molecule nano-flow cell

2007

A Orte, TD Craggs, SS White, SE Jackson, D Klenerman

BIOPHYSICAL JOURNAL

Improved scanning ion conductance microscopy (SICM) for live cell imaging

2007

N Johnson, C Li, V Ostanin, A Bruckbauer, Y Korchev, D Klenerman

BIOPHYSICAL JOURNAL

Single molecule studies of the stoichiometry of human telomerase complex

2007

R Codrington, D Alves, H Li, A Orte, XJ Ren, LM Ying, D Klenerman, S Balasubramanian

BIOPHYSICAL JOURNAL

Basolateral P2X<inf>4</inf>-like receptors regulate the extracellular ATP-stimulated epithelial Na<sup>+</sup> channel activity in renal epithelia

2007

Y Zhang, D Sanchez, J Gorelik, D Klenerman, M Lab, C Edwards, Y Korchev

American Journal of Physiology - Renal Physiology

A Renewable Nanosensor Based on a Glass Nanopipette

2006

JD Piper, RW Clarke, YE Korchev, L Ying, D Klenerman

J Am Chem Soc

A Simple Nanomixer for Single-Molecule Kinetics Measurements

2006

SS White, S Balasubramanian, D Klenerman, L Ying

Angewandte Chemie

Determination of the Fraction and Stoichiometry of Femtomolar Levels of Biomolecular Complexes in an Excess of Monomer Using Single-Molecule, Two-Color Coincidence Detection

2006

A Orte, R Clarke, S Balasubramanian, D Klenerman

Analytical chemistry

Structural analysis of the catalytic core of human telomerase RNA by FRET and molecular modeling

2006

G Gavory, MF Symmons, Y Krishnan Ghosh, D Klenerman, S Balasubramanian

Biochemistry

Characterization of a single molecule DNA switch in free solution.

2006

SS White, H Li, RJ Marsh, JD Piper, ND Leonczek, N Nicolaou, AJ Bain, L Ying, D Klenerman

J Am Chem Soc

Investigating a Quadruplex−Ligand Interaction by Unfolding Kinetics

2006

JJ Green, S Ladame, L Ying, D Klenerman, S Balasubramanian

J Am Chem Soc

Potential biomedical applications of the scanned nanopipette.

2006

D Klenerman, Y Korchev

Nanomedicine (Lond)

Evidence for resonance optical trapping of individual fluorophore-labeled antibodies using single molecule fluorescence spectroscopy.

2006

H Li, D Zhou, H Browne, D Klenerman

Journal of the American Chemical Society

Building three-dimensional nanostructures with active enzymes by surface templated layer-by-layer assembly.

2006

S Rauf, D Zhou, C Abell, D Klenerman, D-J Kang

Chemical communications (Cambridge, England)

Analysis of human telomerase activity and function by two color single molecule coincidence fluorescence spectroscopy.

2006

X Ren, H Li, RW Clarke, DA Alves, L Ying, D Klenerman, S Balasubramanian

Journal of the American Chemical Society

Imaging Proteins in Membranes of Living Cells by High-Resolution Scanning Ion Conductance Microscopy

2006

AI Shevchuk, GI Frolenkov, D Sánchez, PS James, N Freedman, MJ Lab, R Jones, D Klenerman, YE Korchev

Angewandte Chemie (International ed. in English)

Macroscopic 2D networks self-assembled from nanometer-sized protein/DNA complexes.

2006

M Manzanera, DJ Frankel, H Li, D Zhou, A Bruckbauer, P Kreutzmann, JM Blackburn, C Abell, T Rayment, D Klenerman, PD Barker

Nano letters

A reversible pH-driven DNA nanoswitch array.

2006

D Liu, A Bruckbauer, C Abell, S Balasubramanian, D-J Kang, D Klenerman, D Zhou

Journal of the American Chemical Society

Nanoscale pipetting for controlled chemistry in small arrayed water droplets using a double-barrel pipet

2006

KT Rodolfa, A Bruckbauer, D Zhou, AI Schevchuk, YE Korchev, D Klenerman

Nano Letters

A simple nanomixer for single-molecule kinetics measurements.

2006

SS White, S Balasubramanian, D Klenerman, L Ying

Angewandte Chemie - International Edition

Two-component graded deposition of biomolecules with a double-barreled nanopipette.

2005

KT Rodolfa, A Bruckbauer, D Zhou, YE Korchev, D Klenerman

Angew Chem Int Ed Engl

Two-Component Graded Deposition of Biomolecules with a Double-Barreled Nanopipette

2005

KT Rodolfa, A Bruckbauer, D Zhou, YE Korchev, D Klenerman

Angewandte Chemie

Aldosterone acts via an ATP autocrine/paracrine system: the Edelman ATP hypothesis revisited.

2005

J Gorelik, Y Zhang, D Sánchez, A Shevchuk, G Frolenkov, M Lab, D Klenerman, C Edwards, Y Korchev

Proceedings of the National Academy of Sciences of the United States of America

Fluorescence resonance energy transfer between a quantum dot donor and a dye acceptor attached to DNA

2005

D Zhou, JD Piper, C Abell, D Klenerman, D-J Kang, L Ying

Chem Commun (Camb)

Scanning ion conductance microscopy reveals how a functional renal epithelial monolayer maintains its integrity

2005

Y Zhang, J Gorelik, D Sanchez, A Shevchuk, M Lab, I Vodyanoy, D Klenerman, C Edwards, Y Korchev

Kidney Int

The scanned nanopipette: A new tool for high resolution bioimaging and controlled deposition of biomolecules

2005

L Ying, A Bruckbauer, D Zhou, J Gorelik, A Shevchuk, M Lab, Y Korchev, D Klenerman

Physical Chemistry Chemical Physics

Trapping of Proteins under Physiological Conditions in a Nanopipette

2005

RW Clarke, SS White, D Zhou, L Ying, D Klenerman

Angew Chem Int Ed Engl

Fabrication of three-dimensional surface structures with highly fluorescent quantum dots by surface-templated layer-by-layer assembly

2005

DJ Zhou, A Bruckbauer, C Abell, D Klenerman, DJ Kang

Advanced Materials

Aldosterone acts via an ATP autocrine/paracrine system: the Edelman ATP hypothesis revisited.

2005

J Gorelik, Y Zhang, D Sánchez, A Shevchuk, G Frolenkov, M Lab, D Klenerman, C Edwards, Y Korchev

Proceedings of the National Academy of Sciences of the United States of America

Individual Molecules of Dye-Labeled DNA Act as a Reversible Two-Color Switch upon Application of an Electric Field

2004

SS White, L Ying, S Balasubramanian, D Klenerman

Angewandte Chemie

Influence of the Foundation Layer on the Layer-by-Layer Assembly of Poly-l-lysine and Poly(styrenesulfonate) and Its Usage in the Fabrication of 3D Microscale Features

2004

D Zhou, A Bruckbauer, M Batchelor, D-J Kang, C Abell, D Klenerman

Langmuir

Measuring single-molecule nucleic acid dynamics in solution by two-color filtered ratiometric fluorescence correlation spectroscopy.

2004

H Li, X Ren, L Ying, S Balasubramanian, D Klenerman

Proceedings of the National Academy of Sciences of the United States of America

A simple voltage controlled enzymatic nanoreactor produced in the tip of a nanopipet

2004

A Bruckbauer, DJ Zhou, LM Ying, C Abell, D Klenerman

Nano Letters

Molecule by molecule direct and quantitative counting of antibody-protein complexes in solution

2004

H Li, D Zhou, H Browne, S Balasubramanian, D Klenerman

Analytical Chemistry

An addressable antibody nanoarray produced on a nanostructured surface.

2004

A Bruckbauer, D Zhou, D-J Kang, YE Korchev, C Abell, D Klenerman

Journal of the American Chemical Society

The use of scanning ion conductance microscopy to image A6 cells

2004

J Gorelik, Y Zhang, AI Shevchuk, GI Frolenkov, D Sánchez, MJ Lab, I Vodyanoy, CRW Edwards, D Klenerman, YE Korchev

Molecular and Cellular Endocrinology

Frequency and voltage dependence of the dielectrophoretic trapping of short lengths of DNA and dCTP in a nanopipette

2004

L Ying, SS White, A Bruckbauer, L Meadows, YE Korchev, D Klenerman

Biophys J

Evaluation of the effect of carbon dioxide corrosion inhibitors on cavitation damage caused during the ultrasound test

2004

AG Petersen, D Klenerman, WM Hedges

Corrosion

The use of scanning ion conductance microscopy to image A6 cells

2004

J Gorelik, Y Zhang, AI Shevchuk, GI Frolenkov, D Sánchez, MJ Lab, I Vodyanoy, CRW Edwards, D Klenerman, YE Korchev

Molecular and Cellular Endocrinology

Individual molecules of dye-labeled DNA act as a reversible two-color switch upon application of an electric field.

2004

SS White, L Ying, S Balasubramanian, D Klenerman

Angew Chem Int Ed Engl

Studies on the structure and dynamics of the human telomeric G quadruplex by single-molecule fluorescence resonance energy transfer.

2003

LM Ying, JJ Green, HT Li, D Klenerman, S Balasubramanian

Proceedings of the National Academy of Sciences of the United States of America

Studies on the structure and dynamics of the human telomeric G quadruplex by single-molecule fluorescence resonance energy transfer

2003

L Ying, JJ Green, H Li, D Klenerman, S Balasubramanian

Proc Natl Acad Sci U S A

Identification of a new RNA.RNA interaction site for human telomerase RNA (hTR): structural implications for hTR accumulation and a dyskeratosis congenita point mutation.

2003

X Ren, G Gavory, H Li, L Ying, D Klenerman, S Balasubramanian

Nucleic acids research

Building Three-Dimensional Surface Biological Assemblies on the Nanometer Scale

2003

D Zhou, A Bruckbauer, LM Ying, C Abell, D Klenerman

Nano Letters

Multicomponent submicron features of biomolecules created by voltage controlled deposition from a nanopipet

2003

A Bruckbauer, D Zhou, L Ying, YE Korchev, C Abell, D Klenerman

Journal of the American Chemical Society

Dynamic assembly of surface structures in living cells.

2003

J Gorelik, AI Shevchuk, GI Frolenkov, IA Diakonov, MJ Lab, CJ Kros, GP Richardson, I Vodyanoy, CRW Edwards, D Klenerman, YE Korchev

Proceedings of the National Academy of Sciences

Vibrational spectroscopy of mechanically compressed monolayers.

2003

O Berg, D Klenerman

Journal of the American Chemical Society

Surface-sensitive molecular information on polymers from UV-resonance Raman spectroscopy.

2003

J-H Wang, D Klenerman, A van der Pol, RJ Meier

Appl Spectrosc

Kinetics of Unfolding the Human Telomeric DNA Quadruplex Using a PNA Trap

2003

JJ Green, L Ying, D Klenerman, S Balasubramanian

Journal of the American Chemical Society

Ultrasensitive coincidence fluorescence detection of single DNA molecules.

2003

H Li, L Ying, JJ Green, S Balasubramanian, D Klenerman

Analytical chemistry

A novel light source for SICM-SNOM of living cells.

2003

AM Rothery, J Gorelik, A Bruckbauer, W Yu, YE Korchev, D Klenerman

Journal of microscopy

Surface-sensitive molecular information on polymers from UV-resonance Raman spectroscopy

2003

JH Wang, D Klenerman, A Van Der Pol, RJ Meier

Applied Spectroscopy

Scanning surface confocal microscopy for simultaneous topographical and fluorescence imaging: Application to single virus-like particle entry into a cell

2002

J Gorelik, A Shevchuk, M Ramalho, M Elliott, C Lei, CF Higgins, MJ Lab, D Klenerman, N Krauzewicz, Y Korchev

Proceedings of the National Academy of Sciences

Ion channels in small cells and subcellular structures can be studied with a smart patch-clamp system.

2002

J Gorelik, Y Gu, HA Spohr, AI Shevchuk, MJ Lab, SE Harding, CRW Edwards, M Whitaker, GWJ Moss, DCH Benton, D Sánchez, A Darszon, I Vodyanoy, D Klenerman, YE Korchev

Biophys J

Writing with DNA and protein using a nanopipet for controlled delivery

2002

A Bruckbauer, L Ying, AM Rothery, D Zhou, AI Shevchuk, C Abell, YE Korchev, D Klenerman

J Am Chem Soc

Characterization of a Novel Light Source for Simultaneous Optical and Scanning Ion Conductance Microscopy

2002

A Bruckbauer, L Ying, AM Rothery, YE Korchev, D Klenerman

Anal Chem

High-resolution scanning patch-clamp: new insights into cell function

2002

Y Gu, J Gorelik, HA Spohr, A Shevchuk, MJ Lab, SE Harding, I Vodyanoy, D Klenerman, YE Korchev

The FASEB journal : official publication of the Federation of American Societies for Experimental Biology

Single-molecule FRET study of DNA G-quadruplex

2002

J Green, LM Ying, D Klenerman, S Blasubramanian

Chemical Research in Chinese Universities

Programmable Delivery of DNA through a Nanopipet

2002

L Ying, A Bruckbauer, AM Rothery, YE Korchev, D Klenerman

Analytical chemistry

Effect of Cavitation on Carbon Dioxide Corrosion and the Development of a Test for Evaluating Inhibitors

2002

AG Petersen, D Klenerman, WM Hedges, ML Harris

Corrosion

A Sensitive and Economical Method to Directly Detect Particles

2002

MA Cooper, VP Ostanin, D Klenerman, A Slepstov, R Karamanska, FN Dultsev, K Stirrups, S Kelling, T Minson, C Abell

Proceedings of IEEE Sensors

Effects of mechanical compression on the vibrational spectrum of a self-assembled monolayer

2001

O Berg, D Klenerman

Journal of Applied Physics

Direct and sensitive detection of a human virus by rupture event scanning.

2001

MA Cooper, FN Dultsev, T Minson, VP Ostanin, C Abell, D Klenerman

Nature Biotechnology

Simultaneous Measurement of Ca2+ and Cellular Dynamics: Combined Scanning Ion Conductance and Optical Microscopy to Study Contracting Cardiac Myocytes

2001

AI Shevchuk, J Gorelik, SE Harding, MJ Lab, D Klenerman, YE Korchev

Biophys J

Direct and Quantitative Detection of Bacteriophage by “Hearing” Surface Detachment Using a Quartz Crystal Microbalance

2001

FN Dultsev, RE Speight, MT Fiorini, JM Blackburn, C Abell, VP Ostanin, D Klenerman

Analytical chemistry

Non-arrhenius kinetics for the loop closure of a DNA hairpin

2001

MI Wallace, L Ying, S Balasubramanian, D Klenerman

Proceedings of the National Academy of Sciences of the United States of America

Probing DNA surface attachment and local environment using single molecule spectroscopy

2001

MA Osborne, CL Barnes, S Balasubramanian, D Klenerman

The Journal of Physical Chemistry B

Two-state model of conformational fluctuation in a DNA hairpin-loop

2001

LM Ying, MI Wallace, D Klenerman

Chemical Physics Letters

Effect of ultrasound on CO2 corrosion and the development of a slug flow simulator

2001

AG Petersen, D Klenerman, B Hedges, L Harris

NACE - International Corrosion Conference Series

FRET Fluctuation Spectroscopy:  Exploring the Conformational Dynamics of a DNA Hairpin Loop

2000

MI Wallace, LM Ying, S Balasubramanian, D Klenerman

The Journal of Physical Chemistry B

In-situ studies of polycrystalline copper during methanol synthesis at high pressure using sum frequency generation at surfaces.

2000

SJ Lin, A Oldfield, D Klenerman

Surface Science

Single molecule analysis of DNA immobilized on microspheres

2000

MA Osborne, WS Furey, D Klenerman, S Balasubramanian

Anal Chem

Ratiometric Analysis of Single-Molecule Fluorescence Resonance Energy Transfer Using Logical Combinations of Threshold Criteria: A Study of 12-mer DNA

2000

LM Ying, MI Wallace, S Balasubramanian, D Klenerman

The Journal of Physical Chemistry B

“Hearing” Bond Breakage. Measurement of Bond Rupture Forces Using a Quartz Crystal Microbalance

2000

FN Dultsev, VP Ostanin, D Klenerman

Langmuir

Hybrid scanning ion conductance and scanning near-field optical microscopy for the study of living cells.

2000

YE Korchev, M Raval, MJ Lab, J Gorelik, CR Edwards, T Rayment, D Klenerman

Biophys J

Model corrosion inhibitor for in situ spectroscopic studies

2000

B Heeg, D Klenerman

Langmuir

Conformational heterogeneity of dye labelled DNA: Ensemble fluorescence lifetimes and the ratiometric analysis of single-molecule fluorescence resonance energy transfer.

2000

LM Ying, MI Wallace, S Balasubramanian, D Klenerman

BIOPHYS J

Measurements of the Adsorption Kinetics of a Cationic Surfactant in a Liquid Jet by Ellipsometry

1999

J Hutchison, D Klenerman, S Manning-Benson, C Bain

Langmuir

Surface vibrational spectroscopy of lubricants adsorbed at the iron-water interface

1998

DC Duffy, A Friedmann, SA Boggis, D Klenerman

Langmuir

Persistency of corrosion inhibitor films on C-steel under multiphase flow conditions. Part II: optical SHG and electrochemical studies

1998

B Heeg, D Klenerman

Corrosion Science

Ultraviolet Resonance Raman Study of Drug Binding in Dihydrofolate Reductase, Gyrase, and Catechol O-Methyltransferase

1998

VW Couling, P Fischer, D Klenerman, W Huber

Biophysical journal

Persistency of corrosion inhibitor films on C-steel under multiphase flow conditions. Part I: the jet-cylinder arrangement

1998

B Heeg, T Moros, D Klenerman

Corrosion Science

Optically Biased Diffusion of Single Molecules Studied by Confocal Fluorescence Microscopy

1998

MA Osborne, S Balasubramanian, WS Furey, D Klenerman

The Journal of Physical Chemistry B

Optical chemical imaging of tobacco mosaic virus in solution at 60-nm resolution

1998

TH Keller, T Rayment, D Klenerman

Biophysical journal

Use of fluorescence resonance energy transfer to investigate the conformation of DNA substrates bound to the Klenow fragment.

1998

WS Furey, CM Joyce, MA Osborne, D Klenerman, JA Peliska, S Balasubramanian

Biochemistry

Scanning near-field optical microscopy in reflection mode imaging in liquid

1997

TH Keller, T Rayment, D Klenerman, RJ Stephenson

Review of Scientific Instruments

Ultraviolet resonance Raman spectroscopic study of the average environment of tyrosine in native and denatured barnase

1997

VW Couling, NW Foster, D Klenerman

Journal of Raman Spectroscopy

Mechanistic studies of the corrosion inhibitor oleic imidazoline

1994

A EDWARDS, C OSBORNE, S WEBSTER, D KLENERMAN, M JOSEPH, P OSTOVAR, M DOYLE

Corrosion Science

2ND-HARMONIC GENERATION AT SURFACES APPLIED TO CORROSION INHIBITION

1994

D KLENERMAN, J HODGE, M JOSEPH

Corrosion Science

Sum frequency spectra in the C-H stretch region of adsorbates on iron

1993

CR MAECHLING, DAV KLINER, D KLENERMAN

Applied Spectroscopy

A 2ND-HARMONIC GENERATION STUDY OF A CORROSION-INHIBITOR ON A MILD-STEEL ELECTRODE

1992

M JOSEPH, D KLENERMAN

Journal of Electroanalytical Chemistry

UV resonance Raman spectroscopy using a quasi–continuous—wave laser developed as an industrial analytical technique

1992

KPJ WILLIAMS, D KLENERMAN

Journal of Raman Spectroscopy

Multiplexed optically heterodyned Raman-induced Kerr-effect spectroscopy in the blue.

1991

D Klenerman

Opt Lett

Optically heterodyned Raman-induced Kerr-effect spectroscopy using a white-light continuum

1990

D KLENERMAN, DL GERRARD, H HERMAN, MT MACPHERSON

Chemical Physics Letters

Overtone spectra of C–H oscillators in cold molecules

1988

MW CROFTON, CG STEVENS, D KLENERMAN, JH GUTOW, RN ZARE

The Journal of Chemical Physics

Comparison of overtone-induced and electronic photochemistry of liquid tert-butyl hydroperoxide: supporting evidence for vibrational mode selectivity

1988

JH GUTOW, D KLENERMAN, RN ZARE

Journal of Physical Chemistry

Infrared chemiluminescence studies using a SISAM spectrometer. Reactions producing vibrationally excited OH

1987

D KLENERMAN, IWM SMITH

Journal of the Chemical Society, Faraday Transactions 2: Molecular and Chemical Physics

Infrared chemiluminescence using a SISAM spectrometer. Reactions producing vibrationally excited HF

1987

D KLENERMAN, IWM SMITH

Journal of the Chemical Society, Faraday Transactions 2

HOW EFFECTIVE IS INTERNAL EXCITATION IN PROMOTING THE HCL+1,3-BUTADIENE ADDITION-REACTION

1986

D KLENERMAN, RN ZARE

Chemical Physics Letters